tissue culture

Learn to propagate plants using micropropagation techniques in a laboratory.

Tissue culture involves growing plants from very small sections (sometimes microscopic) in a laboratory. It is a propagation method which is being increasingly used. While not appropriate for all plants, for specialist crops such as orchids, some indoor plants and many new plant varieties, it is a very popular propagation method.

As with all our courses, study material is provided on a CD, in total involving approximately 100 hours of work. The course is divided into 9 lessons with an assignment set at the end of each. These are sent to the Academy for marking and are returned to you with comments and suggestions.

You will be working with Colin Elliott, an experienced professional horticulturist.

Course content:

  • Introduction including a review of basic plant nutrition.
    • Stages in tissue cultured plant development
    • Introduction to Plant Growth Science, biochemical processes and cell biology
    • Transpiration, Photosynthesis and Respiration
    • Plant Parts -Stems, Leaves, Roots, Buds,Flowers and fruits
    • What happens as Tissue Matures
    • Types of Plant Tissue
    • Methods of Shoot Induction and Proliferation
    • Advantitious Roots
    • Terminology
  • Plant Nutrients
    • Major Elements
    • Minor (Trace) Elements
    • Total Salts
    • How Plants Grow
    • Factors Affecting Nutrient Uptake
    • Nutrient Solution Preparation
    • Hydroponic Nutrients
    • Chelates
    • Growing Media for Tissue Culture
    • Water in Tissue Culture
    • Chemical Analysis
  • The Laboratory
    • The Tissue Culture Laboratory
    • Preparation Area
    • Transfer Chamber
    • Culture Growing Area
    • Siting a New Lab
    • Equipment Requirements for a Lab
    • Chemicals
  • Micropropagation Techniques
    • Stock Plants -selection, planting, management
    • Uses for Tissue Culture
    • Problems with Tissue Culture
    • Procedures
    • Explants
    • Sterilisation
    • Nutrient Media
    • Shoot Induction and Proliferation
    • Rooting and Planting Out
    • Stages in Plant Development
    • Treating Plant Tissue with Sterilants
  • Plant Hormones
    • Chemical Growth Modification
    • Principles of Using Plant Hormones
    • Auxins, Cytokinins, Gibberellins, Abscisic acid and Ethylene.
    • Other Chemical Treatments
  • The Tissue Culture Environment
    • Media Types -Filter Bridge, Agar, Liquid
    • Nutrient Media Composition
    • Cleanlines
    • Light and Temperature
    • Hormones
    • Artificial Light
    • Water Quality
    • Water Treatgments
    • Carbon Dioxide Effects
    • Greenhouses
    • Diagnosis of Plant Disorders
  • Commercial Applications
    • Understanding Genetics and Plant Breeding
    • Biotechnology
    • Cell Fusions
    • Overcoming Pollination Incompatibility
    • Pollination Biology
  • Taking Plants out of Culture
    • Hardening off Plants
    • Growing Rooms or Chambers
    • Rockwool Applications with Micropropagation
  • Culture of Selected Species
    • Begonia
    • Cattleya
    • Cymbidium
    • Review of a range of other plants
     

Course Aims

  • Explain the nature of plant growth processes, in the tissue culture environment.
  • Determine growing media to use for tissue culture.
  • Specify appropriate micropropagation procedures for different purposes.
  • Explain the management of environmental control equipment used in tissue culture.
  • Design a layout for a commercial tissue culture facility.
  • Determine appropriate commercial applications for tissue culture.

You will learn a great deal from this course through a combination of reading, interacting with tutors, undertaking research and practical tasks. For those looking for information leading to employment in this exciting new field, this is an ideal starting point.

Course cost: £ 380.00 (single payment)

Payment Method

 

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